| Field | Specification |
|---|---|
| Target | |
| Alternative names | Krueppel-like factor 10; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1; TGFB-inducible early growth response protein 1; TIEG-1; KLF10; TIEG; TIEG1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KLF10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22K32 is a rabbit monoclonal antibody that recognizes krueppel-like factor 10 (gene KLF10) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 52.6 kDa, and the supplier reports an observed band at about 53 kDa.
KLF10 (TIEG1) is a Krüppel-like zinc finger transcription factor that is rapidly induced by TGF-β. It mostly represses target genes, extending TGF-β responses, and it also takes part in circadian control of hepatic glucose and lipid metabolism by repressing BMAL1 and gluconeogenic genes. KLF10 is studied in TGF-β signaling, bone biology, metabolism and immune regulation.
| Target | Krueppel-like factor 10 (gene KLF10; UniProt Q13118, human) |
|---|---|
| Clone | 22K32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.6 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells, NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KLF10.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places krueppel-like factor 10 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Langlang Zheng et al. (2020) KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression. ACTA Histochemica. 10.1016/j.acthis.2020.151528
