| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glandular kallikrein-7, submandibular/renal; rGK-7; Esterase B; Kallikrein-related protein K1; Proteinase A; RSKG-7; Tissue kallikrein; Klk7; Klk-7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Klk7 recombinant protein (Position: K30-N238). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets glandular kallikrein-7, submandibular/renal (gene Klk7) in rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 29.0 kDa, and the supplier reports an observed band at about 38 kDa.
In the rat, this glandular kallikrein (rat Klk7, a true tissue kallikrein) is a serine protease that cleaves kininogen to release Lys-bradykinin. It is the main kallikrein produced in the rat kidney and is also found in the submandibular gland, where it contributes to the kallikrein–kinin system that regulates vascular tone and renal function.
| Target | Glandular kallikrein-7, submandibular/renal (gene Klk7; UniProt P36373, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 29.0 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
| ELISA | 0.1–0.5 µg/mL (Rat) |
Samples with a confirmed band (WB): rat kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant rat Klk7 fragment (Lys30–Asn238), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat. Tissue expression noted by UniProt (rat): Kidney and submandibular gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
