| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone-lysine N-methyltransferase, H3 lysine-79 specific;2.1.1.43;DOT1-like protein;Histone H3-K79 methyltransferase;H3-K79-HMTase;Lysine N-methyltransferase 4;DOT1L;KIAA1814, KMT4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human KMT4 / Dot1L |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DOT1L antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone CBF-4; isotype Rabbit IgG; reactivity: Human,Mouse. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody catalog # M00840. Tested in IHC, WB application. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: DOT1L (Histone-lysine N-methyltransferase, H3 lysine-79 specific).
- Antibody format: Monoclonal; clone CBF-4; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DOT1L (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. Reported cellular localization context: Nucleus . Tissue expression notes (as provided): KMT4 / Dot1L Antibody detects endogenous levels of total KMT4 / Dot1L
Research relevance and current trends
- Research context keywords from the source record include: Chromatin Modifying Enzymes,DNA/RNA,DNA Damage & Repair,Epigenetics and Nuclear Signaling,Methylation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate DOT1L antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DOT1L expression by Western blot in cell or tissue lysates, Detect DOT1L in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 68 kDa; calculated MW: 184853 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 68 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): KMT4 / Dot1L Antibody detects endogenous levels of total KMT4 / Dot1L
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
