| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone-lysine N-methyltransferase, H3 lysine-79 specific;2.1.1.43;DOT1-like protein;Histone H3-K79 methyltransferase;H3-K79-HMTase;Lysine N-methyltransferase 4;DOT1L;KIAA1814, KMT4; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KMT4 / Dot1L |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CBF-4 is a rabbit monoclonal antibody that recognizes histone-lysine N-methyltransferase, H3 lysine-79 specific (gene DOT1L) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 164.9 kDa, and the supplier reports an observed band at about 185 kDa.
DOT1L is a histone methyltransferase that methylates lysine 79 of histone H3 (H3K79), a mark associated with actively transcribed genes. Unlike most histone lysine methyltransferases, it lacks a SET domain. DOT1L is required by MLL-rearranged leukemias for aberrant gene expression and is studied in transcription, development and leukemia therapy research.
| Target | Histone-lysine N-methyltransferase, H3 lysine-79 specific (gene DOT1L; UniProt Q8TEK3, human) |
|---|---|
| Clone | CBF-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 164.9 kDa |
| Observed MW | 185 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human KMT4 / Dot1L.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places histone-lysine N-methyltransferase, H3 lysine-79 specific in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
