| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GTPase NRas;Transforming protein N-Ras;NRAS;HRAS1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human KRAS+HRAS+NRAS |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-NRAS antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone EBE-14; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody catalog # M00099-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: NRAS (GTPase NRas).
- Antibody format: Monoclonal; clone EBE-14; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NRAS (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Reported cellular localization context: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus. Tissue expression notes (as provided): Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Susceptibility,Epigenetics and Nuclear Signaling,G Protein Signaling,Proto-Oncogenes,Ras Family,Signal Transduction,Signaling Pathway,Small G Proteins,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate NRAS antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NRAS expression by Western blot in cell or tissue lysates, Localize NRAS by immunofluorescence/immunocytochemistry in cultured cells, Quantify NRAS-positive cells by flow cytometry in single-cell suspensions, Enrich NRAS by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 21 kDa; calculated MW: 21229 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 21 kDa
- Cellular localization (provided): Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus.
- Tissue details (provided): Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
