| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium voltage-gated channel subfamily B member 1 ;Delayed rectifier potassium channel 1 ;DRK1 ;h-DRK1 ;Voltage-gated potassium channel subunit Kv2.1 ;KCNB1 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Kv2.1 recombinant protein (Position: V687-I858). Human Kv2.1 shares 88% amino acid (aa) sequence identity with both mouse and rat Kv2.1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes potassium voltage-gated channel subfamily B member 1 (gene KCNB1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 95.9 kDa, and the supplier reports an observed band at about 96 kDa.
Kv2.1 (KCNB1) is a voltage-gated potassium channel that carries a major delayed-rectifier current in neurons, and it is also present in pancreatic β-cells and the cardiovascular system. It helps repolarize action potentials and limits high-frequency firing, and it clusters on neuronal cell bodies where it also supports exocytosis. KCNB1 variants are linked to developmental and epileptic encephalopathy.
| Target | Potassium voltage-gated channel subfamily B member 1 (gene KCNB1; UniProt Q14721, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 95.9 kDa |
| Observed MW | 96 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, rat brain tissue, mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): rat brain tissue, mouse brain tissue.
Immunogen
Recombinant human Kv2.1 fragment (Val687–Ile858), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 88%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places potassium voltage-gated channel subfamily B member 1 in the cell membrane, perikaryon, cell projection and membrane. Tissue expression noted by UniProt (human): Expressed in neocortical pyramidal cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
