| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium voltage-gated channel subfamily D member 3;Voltage-gated potassium channel subunit Kv4.3;KCND3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Kv4.3 recombinant protein (Position: M1-H177). Human Kv4.3 shares 100% and 99% amino acid (aa) sequences identity with mouse and rat Kv4.3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets A-type voltage-gated potassium channel KCND3 in human and mouse samples and is validated for Western blot. The predicted molecular weight is 73.5 kDa, and the supplier reports an observed band at about 73 kDa.
Kv4.3 (KCND3) is the pore-forming subunit of a Shal-type A-type potassium channel expressed in brain and heart. In cardiac muscle it contributes to the transient outward current (Ito) that shapes early repolarization, and in neurons it conducts a subthreshold somatodendritic A-current. Its activation and inactivation are tuned by partners such as KChIP2 and DPP6, and KCND3 variants are linked to spinocerebellar ataxia and Brugada syndrome.
| Target | A-type voltage-gated potassium channel KCND3 (gene KCND3; UniProt Q9UK17, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 73.5 kDa |
| Observed MW | 73 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Human) |
|---|
Samples with a confirmed band (WB): mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human Kv4.3 fragment (Met1–His177), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100% and 99%, respectively.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places A-type voltage-gated potassium channel KCND3 in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Liu et al. (2022) Exogenous H2S promotes ion channel reconstruction to regulate colonic motility in rats with dinitrobenzene sulfonic acid-induced colitis. Annals of Translational Medicine. 10.21037/atm-22-2126
Ayse Karatug Kacar (2023) Exploring dual effects of dinutuximab beta on cell death and proliferation of insulinoma. Chemical Biology & Drug Design. 10.1111/cbdd.14368
