| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-17 receptor A;IL-17 receptor A;IL-17RA;CDw217;CD217;IL17RA;IL17R; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL17RA recombinant protein (Position: K53-Q284). Human IL17RA shares 74.1% amino acid (aa) sequence identity with mouse IL17RA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interleukin-17 receptor A (gene IL17RA) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 96.1 kDa, and the supplier reports an observed band at about 120–150 kDa.
IL17RA is a receptor subunit shared by several IL-17 family cytokines. Paired with IL17RC it binds IL-17A and IL-17F, and through its SEFIR domain it recruits the adaptor ACT1 (TRAF3IP2) to activate NF-κB and MAPK pathways, making it a central receptor in Th17-mediated inflammation.
| Target | Interleukin-17 receptor A (gene IL17RA; UniProt Q96F46, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 96.1 kDa |
| Observed MW | 120–150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human MOLT-4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human IL17RA fragment (Lys53–Gln284), expressed in E. coli. Sequence identity with the mouse ortholog: 74.1%.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
