| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human L1CAM, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat L1CAM, respectively. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets neural cell adhesion molecule L1 (gene L1CAM) in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 140.0 kDa.
L1CAM is a transmembrane cell adhesion molecule of the immunoglobulin superfamily that is expressed mainly in the nervous system. It mediates adhesion between neurons and signals to the cytoskeleton and growth factor receptors, supporting neuronal migration, axon growth and bundling, and synapse formation. Mutations in L1CAM cause L1 syndrome, which includes X-linked hydrocephalus, and it is also studied in cancer metastasis.
| Target | Neural cell adhesion molecule L1 (gene L1CAM; UniProt P32004, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 140.0 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human colon cancer tissue, human glioma tissue, human lung cancer tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the middle region of human L1CAM. Sequence identity with the mouse and rat orthologs: 88.2% and 82.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neural cell adhesion molecule L1 in the cell membrane and cell projection.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Manule Y et al. (2023) Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma. Asian Pacific Journal of Cancer Prevention. 10.31557/apjcp.2023.24.4.1413