| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human L1CAM, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat L1CAM, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of L1CAM in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-L1CAM Antibody Picoband® catalog # A00729-1. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human L1CAM, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat L1CAM, respectively.
- Molecular weight context: reported MW: 52 kDa, 60 kDa; calculated MW: nan
- Reactivity: Human,Mouse,Rat
- Applications: IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
L1 cell adhesion molecule. L1, also known as L1CAM, is a transmembrane protein member of the L1 protein family, encoded by the L1CAM gene. The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. Functional note: Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity. Reported localization: Cell membrane. Expression/tissue context: Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Research relevance and current trends
- Axonal Guidance Proteins: Researchers commonly examine how L1CAM relates to this theme using model systems and orthogonal readouts.
- Cell Adhesion: Researchers commonly examine how L1CAM relates to this theme using model systems and orthogonal readouts.
- Cell Adhesion Molecules: Researchers commonly examine how L1CAM relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of L1CAM across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
