Anti-L1CAM Antibody

SKU:BHA21001576
Research Validated
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Overview
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Anti-L1CAM antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Human, Mouse, Rat. Recognizes neural cell adhesion molecule L1. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target L1CAM
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
A00729-1 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IHC
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
      Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target L1CAM
Alternative names Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5
UniProt # P32004
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human L1CAM, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat L1CAM, respectively.
Molecular weight 140.0 kDa
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Cell membrane.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A00729-1
Main SKU BHA21001576
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody targets neural cell adhesion molecule L1 (gene L1CAM) in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 140.0 kDa.

L1CAM is a transmembrane cell adhesion molecule of the immunoglobulin superfamily that is expressed mainly in the nervous system. It mediates adhesion between neurons and signals to the cytoskeleton and growth factor receptors, supporting neuronal migration, axon growth and bundling, and synapse formation. Mutations in L1CAM cause L1 syndrome, which includes X-linked hydrocephalus, and it is also studied in cancer metastasis.

Target Neural cell adhesion molecule L1 (gene L1CAM; UniProt P32004, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 140.0 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 0.5–1 µg/mL

Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human colon cancer tissue, human glioma tissue, human lung cancer tissue, human mammary cancer tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).

Immunogen

Synthetic peptide from the middle region of human L1CAM. Sequence identity with the mouse and rat orthologs: 88.2% and 82.4%, respectively.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places neural cell adhesion molecule L1 in the cell membrane and cell projection.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 0.5–1 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.Has this antibody been tested on goat samples?
A.Reactivity with goat samples has not been tested by the supplier. Aligning the immunogen sequence with the goat ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide from the middle region of human L1CAM. Sequence identity with the mouse and rat orthologs: 88.2% and 82.4%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Manule Y et al. (2023) Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma. Asian Pacific Journal of Cancer Prevention. 10.31557/apjcp.2023.24.4.1413

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