| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neural cell adhesion molecule L1;N-CAM-L1;NCAM-L1;CD171;L1CAM;CAML1, MIC5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human L1CAM |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-L1CAM antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAFD-12; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-L1CAM Rabbit Monoclonal Antibody catalog # M00729. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: L1CAM (Neural cell adhesion molecule L1).
- Antibody format: Monoclonal; clone AAFD-12; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
L1CAM (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Reported cellular localization context: Cell membrane ; Single-pass type I membrane protein . Cell projection, growth cone . Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons. . Tissue expression notes (as provided): Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Research relevance and current trends
- Research context keywords from the source record include: Axonal Guidance Proteins,Cell Adhesion,Cell Adhesion Molecules,Cytoskeleton/ECM,Growth and Development,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate L1CAM antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect L1CAM expression by Western blot in cell or tissue lysates, Detect L1CAM in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 90 kDa; calculated MW: 140003 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 90 kDa
- Cellular localization (provided): Cell membrane ; Single-pass type I membrane protein . Cell projection, growth cone . Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons. .
- Tissue details (provided): Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
