| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lamin-B receptor; Integral nuclear envelope inner membrane protein; LMN2R; LBR |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Lamin B Receptor/LBR recombinant protein (Position: H102-F209). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Lamin B Receptor/LBR Antibody Picoband® is an antibody for LBR detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: LBR (lamin B receptor); UniProt: Q14739
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 65-71 kDa, calculated 123799 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Lamin B Receptor/LBR Antibody Picoband® catalog # A01238-2.
Biological background
Biological context: Anchors the lamina and the heterochromatin to the inner nuclear membrane.
Expression and localization notes: cellular localization: Nucleus inner membrane. Multi-pass membrane protein., tissue context: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow..
Common research applications
- Western blotting (WB): Compare LBR levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of LBR in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify LBR-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Lamin-B receptor is a protein, and in humans, it is encoded by the LBR gene. It is mapped to 1q42.12. The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus inner membrane. Multi-pass membrane protein.
- Tissue details: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.
- Research category: Amino Acid Metabolism,Amino Acids,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
