| Field | Specification |
|---|---|
| Target | |
| Alternative names | Laminin subunit gamma-1;Laminin B2 chain;Laminin-1 subunit gamma;Laminin-10 subunit gamma;Laminin-11 subunit gamma;Laminin-2 subunit gamma;Laminin-3 subunit gamma;Laminin-4 subunit gamma;Laminin-6 subunit gamma;Laminin-7 subunit gamma;Laminin-8 subunit gamma;Laminin-9 subunit gamma;S-laminin subunit gamma;S-LAM gamma;Lamc1;Lamb-2, Lamc-1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Human fibroblasts derived. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets laminin subunit alpha-1 (gene LAMA1) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 337.1 kDa, and the supplier reports an observed band at about 200 kDa.
Laminin alpha-1 (LAMA1) is a subunit of laminin-111, one of the first laminins expressed in the embryo and a major component of early basement membranes. By binding integrins and alpha-dystroglycan, it supports cell adhesion, migration and tissue organization, and it takes part in epithelial branching morphogenesis. LAMA1 is studied in development, basement membrane biology and stem cell culture.
| Target | Laminin subunit alpha-1 (gene LAMA1; UniProt P25391, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 337.1 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human placenta tissue, human hcct tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Human fibroblasts derived.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jin Xiaolu et al. (2026) Activation of α7nAChR reduces inflammation and apoptosis, promoting muscle regeneration through the AKT-FOXO1 pathway. Cell Death and Differentiation. 10.1038/s41418-026-01738-1
Suai Zhang et al. (2022) Amniotic Membrane Enhances the Characteristics and Function of Stem Cell-Derived Retinal Pigment Epithelium Sheets by Inhibiting the Epithelial–Mesenchymal Transition. Acta Biomaterialia. 10.1016/j.actbio.2022.07.064
Yanru Ren et al. (2025) Lycium barbarum polysaccharide mitigates high-fat-diet-induced skeletal muscle atrophy by promoting AMPK/PINK1/Parkin-mediated mitophagy. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.140488
Guoqiang Yang et al. (2022) Mesenchymal Stem Cell-Derived Neuron-Like Cell Transplantation Combined with Electroacupuncture Improves Synaptic Plasticity in Rats with Intracerebral Hemorrhage via mTOR/p70S6K Signaling. Stem Cells International. 10.1155/2022/6450527
Jiang Xiaoqian et al. (2023) MSCs overexpressing GDNF restores brain structure and neurological function in rats with intracerebral hemorrhage. Molecular Biomedicine. 10.1186/s43556-023-00159-7
Chenyan Zhang et al. (2024) MiR-495 reverses in the mechanical unloading, random rotating and aging induced muscle atrophy via targeting MyoD and inactivating the Myostatin/TGF-β/Smad3 axis. Archives of Biochemistry and Biophysics. 10.1016/j.abb.2024.110273
