| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysosome-associated membrane glycoprotein 1;LAMP-1;Lysosome-associated membrane protein 1;CD107 antigen-like family member A;CD107a;LAMP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets lysosome-associated membrane glycoprotein 1 (gene LAMP1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 44.9 kDa, and the supplier reports an observed band at about 90–120 kDa (Glycosylation).
LAMP1 (CD107a) is a heavily glycosylated membrane protein that lines the lysosome and is one of the most widely used lysosomal markers. It supports lysosome biogenesis, acidification and autophagy, in part by restraining the proton channel TMEM175. In NK cells and cytotoxic T cells it appears on the surface after degranulation, so CD107a staining is a standard readout of cytotoxic activity.
| Target | Lysosome-associated membrane glycoprotein 1 (gene LAMP1; UniProt P11279, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 44.9 kDa |
| Observed MW | 90–120 kDa (Glycosylation) |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human Caco-2 cells, human HeLa cells, human U2OS cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human LAMP1. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human. UniProt places lysosome-associated membrane glycoprotein 1 in the lysosome membrane, endosome membrane, late endosome membrane and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tingting Wang et al. (2022) Synergistic Lysosomal Impairment and ER Stress Activation for Boosted Autophagy Dysfunction Based on Te Double-Headed Nano-Bullets. Small. 10.1002/smll.202201585
Haiyu Zhang et al. (2022) 3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy. Phytotherapy Research. 10.1002/ptr.7619
Wang Lihong et al. (2018) A pH probe inhibits senescence in mesenchymal stem cells. Stem Cell Research & Therapy. 10.1186/s13287-018-1081-0
Jing-Wen Su et al. (2021) Estrogen protects against acidosis-mediated articular chondrocyte injury by promoting ASIC1a protein degradation. European Journal of Pharmacology. 10.1016/j.ejphar.2021.174381
Yongke Yuan et al. (2026) Lysosomal dysfunction contributes to lead (Pb) and High-Fat Diet (HFD)-induced neuronal apoptosis: a link with cathepsin B. Neurotoxicology. 10.1016/j.neuro.2026.103462
