| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lysosome-associated membrane glycoprotein 2;LAMP-2;Lysosome-associated membrane protein 2;CD107 antigen-like family member B;Lysosomal membrane glycoprotein type B;LGP-B;CD107b;Lamp2;Lamp-2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human LAMP2a |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-LAMP2a Rabbit Monoclonal Antibody is an antibody targeting LAMP2. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: DIE-12; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 75 kDa; calculated MW: 45681 MW.
Boster Bio Anti-LAMP2a Rabbit Monoclonal Antibody catalog # M01573. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: LAMP2 — Lysosome-associated membrane glycoprotein 2
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: DIE-12; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 75 kDa; Calculated: 45681 MW
Biological background
Protein function (datasheet): Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Cellular localization (datasheet): Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Tissue details (datasheet): Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cardiovascular,Cell Death,Energy Metabolism,Energy Transfer Pathways,Hematopoietic Progenitors,Immunology,Metabolic Signaling Pathways,Metabolism,Metabolism Processes,Myeloid,Organelles,Pathways and Processes,Secreted Molecules,Signal Transduction,Stem Cells,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
