Anti-LAR/PTPRF Antibody Picoband®

Overview

This antibody is intended for detection of PTPRF (Receptor-type tyrosine-protein phosphatase F) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-LAR/PTPRF Antibody Picoband® catalog # PB9786. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human LAR, different from the related mouse and rat sequences by four amino acids.
• Molecular weight context: reported MW: 240 kDa; calculated MW: 212879 MW
• Reactivity: Human
• Applications: IHC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Receptor-type tyrosine-protein phosphatase F; Receptor-type tyrosine-protein phosphatase F. Receptor-type tyrosine-protein phosphatase F is an enzyme that in humans is encoded by the PTPRF gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Functional note: Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Expressed in breast, placenta, kidney, liver and pancreas. .

Research relevance and current trends

• Neurology Process: Researchers commonly examine how PTPRF (Receptor-type tyrosine-protein phosphatase F) relates to this theme using model systems and orthogonal readouts.
• Neuron Marker: Researchers commonly examine how PTPRF (Receptor-type tyrosine-protein phosphatase F) relates to this theme using model systems and orthogonal readouts.
• Neuroscience: Researchers commonly examine how PTPRF (Receptor-type tyrosine-protein phosphatase F) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative PTPRF (Receptor-type tyrosine-protein phosphatase F) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of PTPRF (Receptor-type tyrosine-protein phosphatase F) across tissue regions and cell types using matched controls.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.