| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylcholine-sterol acyltransferase;2.3.1.43 ;Lecithin-cholesterol acyltransferase;Phospholipid-cholesterol acyltransferase;Lcat; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse LCAT, different from the related human sequence by six amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes phosphatidylcholine-sterol acyltransferase (gene Lcat) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 49.7 kDa, and the supplier reports an observed band at about 50 kDa.
Lecithin–cholesterol acyltransferase (LCAT) is a plasma enzyme secreted mainly by the liver. On the surface of HDL and LDL particles it transfers a fatty acid from phosphatidylcholine to free cholesterol, forming cholesteryl esters that drive HDL maturation and reverse cholesterol transport. LCAT deficiency causes marked lipoprotein abnormalities, and the enzyme is studied in lipid metabolism and cardiovascular research.
| Target | Phosphatidylcholine-sterol acyltransferase (gene Lcat; UniProt P16301, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 49.7 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse testis tissue, HepG2 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of mouse LCAT. It differs from the human sequence at six residues and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes phosphatidylcholine-sterol acyltransferase as a secreted protein. Tissue expression noted by UniProt (mouse): Detected in blood plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
