| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Low-density lipoprotein receptor; LDL receptor; LDLR |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human LDL Receptor/LDLR recombinant protein (Position: Q35-D843). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-LDL Receptor/LDLR Antibody Picoband® is an antibody for LDLR detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: LDLR (low density lipoprotein receptor); UniProt: P01130
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 130 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-LDL Receptor/LDLR Antibody Picoband® catalog # A00076-2.
Biological background
Biological context: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Expression and localization notes: cellular localization: Lysosome. Golgi apparatus. Cell membrane. Single-pass type I membrane protein. Early endosome. Late endosome. Clathrin-coated pit., tissue context: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads..
Common research applications
- Western blotting (WB): Compare LDLR levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of LDLR in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify LDLR-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: In humans, the LDL receptor protein is encoded by the LDLR gene on chromosome 19. It is mapped to 19p13.2. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Lysosome. Golgi apparatus. Cell membrane. Single-pass type I membrane protein. Early endosome. Late endosome. Clathrin-coated pit.
- Tissue details: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
- Research category: Cancer,Cardiovascular,Cytochromes,Drug Metabolism,Growth Factors/Hormones,Lipases,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
