| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low-density lipoprotein receptor; LDL receptor; LDLR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LDL Receptor/LDLR recombinant protein (Position: Q35-D843). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes low-density lipoprotein receptor (gene LDLR) in human and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 95.4 kDa, and the supplier reports an observed band at about 130 kDa.
The LDL receptor (LDLR) is a cell surface receptor that binds apolipoprotein B- and E-containing lipoproteins, above all LDL, and carries them into cells by clathrin-mediated endocytosis. Hepatic LDLR is the main route for clearing LDL cholesterol from plasma, and LDLR mutations cause familial hypercholesterolemia. It is central to lipid metabolism and cardiovascular research, including studies of PCSK9.
| Target | Low-density lipoprotein receptor (gene LDLR; UniProt P01130, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 95.4 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human U-87MG cells, rat liver tissue, rat lung tissue, rat kidney tissue, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): A431 cells, NRK cells.
Immunogen
Recombinant human LDL Receptor/LDLR fragment (Gln35–Asp843), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places low-density lipoprotein receptor in the cell membrane, membrane, Golgi apparatus and early endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ye Qiang et al. (2013) Difference in LDL Receptor Feedback Regulation in Macrophages and Vascular Smooth Muscle Cells: Foam Cell Transformation Under Inflammatory Stress. Inflammation. 10.1007/s10753-013-9769-x
Ye Qiang et al. (2009) Inflammatory stress increases unmodified LDL uptake via LDL receptor: an alternative pathway for macrophage foam-cell formation. Inflammation Research. 10.1007/s00011-009-0052-4
