| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeat-containing G-protein coupled receptor 5;G-protein coupled receptor 49;G-protein coupled receptor 67;G-protein coupled receptor HG38;LGR5;GPR49, GPR67; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LGR5/GPR49 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CHF-12 is a rabbit monoclonal antibody that recognizes leucine-rich repeat-containing G protein-coupled receptor 5 (gene LGR5) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 100.0 kDa, and the supplier reports an observed band at about 100 kDa.
LGR5 is a leucine-rich repeat G protein-coupled receptor that binds R-spondins and strengthens canonical Wnt signaling, largely by opposing the ubiquitin ligases RNF43 and ZNRF3 that remove Wnt receptors. It marks adult stem cells in the intestinal crypt, hair follicle, stomach and other tissues. LGR5 is a key marker in stem cell biology, organoid culture and colorectal cancer research.
| Target | Leucine-rich repeat-containing G protein-coupled receptor 5 (gene LGR5; UniProt O75473, human) |
|---|---|
| Clone | CHF-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 100.0 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human fetal skeletal muscle.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human LGR5/GPR49.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leucine-rich repeat-containing G protein-coupled receptor 5 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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