| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor ligand superfamily member 14; CD258; Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form; Tnfsf14; Light; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse LIGHT recombinant protein (Position: L59-V239). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Tnfsf14 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-LIGHT/Tnfsf14 Antibody Picoband® catalog # A03516-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse LIGHT recombinant protein (Position: L59-V239). (reported region: L59-V239).
- Molecular weight context: reported MW: 26 kDa; calculated MW: nan
- Reactivity: Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
tumor necrosis factor (ligand) superfamily, member 14. Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. Functional note: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB and stimulates the proliferation of T-cells. Reported localization: Cell membrane ; Single-pass type II membrane protein .
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how Tnfsf14 relates to this theme using model systems and orthogonal readouts.
- Cytokines: Researchers commonly examine how Tnfsf14 relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how Tnfsf14 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Tnfsf14 levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
