| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hepatic triacylglycerol lipase; HL; Hepatic lipase; Lipase member C; Lipc |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived rat LIPC recombinant protein (Position: E39-D494). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-LIPC Antibody Picoband® is an antibody for Lipc detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Lipc (lipase C, hepatic type); UniProt: P07867
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 56 kDa, calculated 47579 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-LIPC Antibody Picoband® catalog # A01020-1.
Biological background
Biological context: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Expression and localization notes: cellular localization: Secreted..
Common research applications
- Western blotting (WB): Compare Lipc levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Lipc in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify Lipc-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Hepatic lipase (HL), also called hepatic triglyceride lipase (HTGL) or LIPC (for lipase, hepatic), is a form of lipase, catalyzing the hydrolysis of triacylglyceride. It is mapped to 8q24. LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Research category: Cell Type Marker,Cell Type Markers,Neurodegenerative Disease,Neurology Process,Neuroscience,Tags & Cell Markers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
