| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lipoprotein lipase;LPL;3.1.1.34;LPL;LIPD; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Lipoprotein lipase |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-LPL antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABDE-12; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Lipoprotein lipase LPL Rabbit Monoclonal Antibody catalog # M00316. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: LPL (Lipoprotein lipase).
- Antibody format: Monoclonal; clone ABDE-12; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
LPL (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). . Reported cellular localization context: Cell membrane ; Lipid-anchor, GPI-anchor . Secreted . Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity). . Tissue expression notes (as provided): Ubiquitously expressed in various human primary cells and tumor cell lines. .
Research relevance and current trends
- Research context keywords from the source record include: Stem Cells.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate LPL antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect LPL expression by Western blot in cell or tissue lysates, Detect LPL in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 41 kDa; calculated MW: 53162 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 41 kDa
- Cellular localization (provided): Cell membrane ; Lipid-anchor, GPI-anchor . Secreted . Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity). .
- Tissue details (provided): Ubiquitously expressed in various human primary cells and tumor cell lines. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
