| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Vitronectin;VN;S-protein;Serum-spreading factor;V75;Vitronectin V65 subunit;Vitronectin V10 subunit;Somatomedin-B;VTN; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human LOX12 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ALOX12 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24A29; isotype IgG; reactivity: Human,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-LOX12 Rabbit Monoclonal Antibody catalog # M02275-1. Tested in WB application. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: ALOX12 (Vitronectin).
- Antibody format: Monoclonal; clone 24A29; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ALOX12 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Reported cellular localization context: Secreted, extracellular space. Tissue expression notes (as provided): Plasma.
Research relevance and current trends
- Research context keywords from the source record include: Immunology,Innate Immunity,Lipid Signaling,Signal Transduction,Signaling Pathway,TLR Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate ALOX12 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ALOX12 expression by Western blot in cell or tissue lysates, Compare relative ALOX12 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 75 kDa; calculated MW: 54306 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 75 kDa
- Cellular localization (provided): Secreted, extracellular space.
- Tissue details (provided): Plasma.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
