| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeats and immunoglobulin-like domains protein 1; LIG-1; Lrig1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse LRIG1, which shares 90% amino acid (aa) sequence identity with human LRIG1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets leucine-rich repeats and immunoglobulin-like domains protein 1 (gene Lrig1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 119.2 kDa, and the supplier reports an observed band at about 145 kDa.
LRIG1 is a transmembrane protein with leucine-rich repeats and immunoglobulin-like domains that acts as a negative feedback regulator of receptor tyrosine kinases such as EGFR. By boosting receptor ubiquitination and speeding their breakdown inside the cell, it limits growth factor signaling, and it is studied in stem cell quiescence, epithelial homeostasis and tumor growth.
| Target | Leucine-rich repeats and immunoglobulin-like domains protein 1 (gene Lrig1; UniProt P70193, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 119.2 kDa |
| Observed MW | 145 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the middle region of mouse LRIG1. Sequence identity with the human ortholog: 90%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leucine-rich repeats and immunoglobulin-like domains protein 1 in the cell membrane. Tissue expression noted by UniProt (mouse): Detected in brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ling Chen et al. (2023) IGF2/IGF2R/Sting signaling as a therapeutic target in DSS-induced ulcerative colitis. European Journal of Pharmacology. 10.1016/j.ejphar.2023.176122
Xiaomin Chen et al. (2024) The effect of airborne particulate matter 2.5 (PM2.5) on meibomian gland. Experimental Eye Research. 10.1016/j.exer.2024.109956
