| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeats and immunoglobulin-like domains protein 3;LIG-3;LRIG3;LIG3;UNQ287/PRO326/PRO335; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human LRIG3, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes leucine-rich repeats and immunoglobulin-like domains protein 3 (gene LRIG3) in human and rat samples and is validated for Western blot. The predicted molecular weight is 123.4 kDa, and the supplier reports an observed band at about 123 kDa.
LRIG3 is a member of the LRIG family of transmembrane proteins with leucine-rich repeats and immunoglobulin-like domains. Work in animal models suggests it helps shape craniofacial structures and the inner ear during embryonic development, including formation of the lateral semicircular canal, possibly by limiting netrin-1 expression in the otic epithelium.
| Target | Leucine-rich repeats and immunoglobulin-like domains protein 3 (gene LRIG3; UniProt Q6UXM1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 123.4 kDa |
| Observed MW | 123 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat testis tissue, HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human LRIG3. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places leucine-rich repeats and immunoglobulin-like domains protein 3 in the cell membrane and cytoplasmic vesicle membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Chen et al. (2019) MiR-449a-5p mediates mitochondrial dysfunction and phenotypic transition by targeting Myc in pulmonary arterial smooth muscle cells. Journal of Molecular Medicine-Jmm. 10.1007/s00109-019-01751-7
