| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low-density lipoprotein receptor-related protein 6; LRP-6; LRP6 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LRP6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21L27 is a rabbit monoclonal antibody that recognizes low-density lipoprotein receptor-related protein 6 (gene LRP6) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 180.4 kDa, and the supplier reports an observed band at about 230 kDa.
LRP6 is a cell-surface coreceptor for canonical Wnt/β-catenin signaling that works together with Frizzled receptors. When Wnt binds, LRP6 is phosphorylated and helps gather Dishevelled and the Axin/GSK3 complex into membrane signalosomes, which blocks β-catenin destruction; it is widely studied in embryonic patterning, bone formation and cancer signaling.
| Target | Low-density lipoprotein receptor-related protein 6 (gene LRP6; UniProt O75581, human) |
|---|---|
| Clone | 21L27 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 180.4 kDa |
| Observed MW | 230 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human LRP6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places low-density lipoprotein receptor-related protein 6 in the cell membrane, endoplasmic reticulum and membrane raft. Tissue expression noted by UniProt (human): Widely coexpressed with LRP5 during embryogenesis and in adult tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yushan Wang et al. (2026) NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties. Bioactive Materials. 10.1016/j.bioactmat.2026.01.006
