| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukotriene A-4 hydrolase; LTA-4 hydrolase; Leukotriene A(4) hydrolase; Tripeptide aminopeptidase LTA4H; LTA4H; LTA4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LTA4H |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22L61 is a rabbit monoclonal antibody that recognizes leukotriene A-4 hydrolase (gene LTA4H) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 69.3 kDa, and the supplier reports an observed band at about 69 kDa.
Leukotriene A4 hydrolase (LTA4H) is a zinc-dependent enzyme with two activities. Its epoxide hydrolase activity converts leukotriene A4 into the neutrophil chemoattractant leukotriene B4, while its aminopeptidase activity breaks down the collagen-derived chemotactic peptide Pro-Gly-Pro, so it can both drive and limit inflammation and is studied in lipid mediator and inflammation research.
| Target | Leukotriene A-4 hydrolase (gene LTA4H; UniProt P09960, human) |
|---|---|
| Clone | 22L61 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.3 kDa |
| Observed MW | 69 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human LTA4H.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leukotriene A-4 hydrolase in the cytoplasm. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
