| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glycine--tRNA ligase;3.6.1.17 ;6.1.1.14 ;Diadenosine tetraphosphate synthetase;AP-4-A synthetase;Glycyl-tRNA synthetase;GlyRS;GARS; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human macroH2A.1 Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-H2AFY antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADDI-8; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-macroH2A.1 H2AFY Monoclonal Antibody catalog # M04635. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: H2AFY (Glycine--tRNA ligase).
- Antibody format: Monoclonal; clone ADDI-8; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
H2AFY (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Catalyzes the attachment of glycine to tRNA (Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by condensation of 2 ATPs. . Reported cellular localization context: Cytoplasm . Mitochondrion . Cell projection, axon . Associated with granules in cultured neuron cells. . Tissue expression notes (as provided): Widely expressed, including brain and spinal cord. .
Research relevance and current trends
- Research context keywords from the source record include: Epigenetics and Nuclear Signaling,2339,Histones.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate H2AFY antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect H2AFY expression by Western blot in cell or tissue lysates, Detect H2AFY in FFPE tissue sections by immunohistochemistry, Localize H2AFY by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 38 kDa; calculated MW: 83166 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 38 kDa
- Cellular localization (provided): Cytoplasm . Mitochondrion . Cell projection, axon . Associated with granules in cultured neuron cells. .
- Tissue details (provided): Widely expressed, including brain and spinal cord. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
