| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycine--tRNA ligase;3.6.1.17 ;6.1.1.14 ;Diadenosine tetraphosphate synthetase;AP-4-A synthetase;Glycyl-tRNA synthetase;GlyRS;GARS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human macroH2A.1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADDI-8 is a rabbit monoclonal antibody that recognizes core histone macro-H2A.1 (gene MACROH2A1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 39.6 kDa, and the supplier reports an observed band at about 40 kDa.
MacroH2A1 is a variant of histone H2A that carries a large C-terminal macrodomain in addition to the histone fold. It replaces standard H2A in a subset of nucleosomes, is enriched on the inactive X chromosome and generally represses transcription; one splice form binds ADP-ribose metabolites, linking chromatin to NAD+ metabolism, and it is studied in epigenetics, differentiation and cancer.
| Target | Core histone macro-H2A.1 (gene MACROH2A1; UniProt O75367, human) |
|---|---|
| Clone | ADDI-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.6 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human macroH2A.1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places core histone macro-H2A.1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
