| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-C motif chemokine 4;G-26 T-lymphocyte-secreted protein;HC21;Lymphocyte activation gene 1 protein;LAG-1;MIP-1-beta (1-69);Macrophage inflammatory protein 1-beta;MIP-1-beta;PAT 744;Protein H400;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2;ACT-2;MIP-1-beta (3-69);CCL4;LAG1, MIP1B, SCYA4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CCL4 recombinant protein (Position: A24-N92). Human CCL4 shares 76.8% and 79.7% amino acid (aa) sequence identity with mouse and rat CCL4, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CCL4 (C-C motif chemokine 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband® catalog # PB9572. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CCL4 recombinant protein (Position: A24-N92). Human CCL4 shares 76.8% and 79.7% amino acid (aa) sequence identity with mouse and rat CCL4, respectively. (reported region: A24-N92).
- Molecular weight context: reported MW: 11 kDa; calculated MW: 10212 MW
- Reactivity: Human
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-C motif chemokine 4; C-C motif chemokine 4. Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. And it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis of CCL4, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14). Functional note: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta (3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. . Reported localization: Secreted. Expression/tissue context: Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Research relevance and current trends
- Immunology: Researchers commonly examine how CCL4 (C-C motif chemokine 4) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how CCL4 (C-C motif chemokine 4) relates to this theme using model systems and orthogonal readouts.
- Macrophage/Inflammation: Researchers commonly examine how CCL4 (C-C motif chemokine 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CCL4 (C-C motif chemokine 4) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the intercrine beta (chemokine CC) family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
