| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic spindle assembly checkpoint protein MAD1;Mitotic arrest deficient 1-like protein 1;MAD1-like protein 1;Mitotic checkpoint MAD1 protein homolog;HsMAD1;hMAD1;Tax-binding protein 181;MAD1L1;MAD1, TXBP181; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitotic spindle assembly checkpoint protein MAD1 (gene MAD1L1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 83.1 kDa, and the supplier reports an observed band at about 83 kDa.
MAD1 (MAD1L1) is a core component of the spindle assembly checkpoint that localizes to unattached kinetochores. Bound to closed MAD2, it acts as a template that converts free MAD2 into its active form, helping generate the mitotic checkpoint complex that delays anaphase until every chromosome is properly attached, so it is studied in chromosome segregation and aneuploidy.
| Target | Mitotic spindle assembly checkpoint protein MAD1 (gene MAD1L1; UniProt Q9Y6D9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 83.1 kDa |
| Observed MW | 83 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human Jurkat cells, human HepG2 whole cellhuman HL-60 whole cellhuman THP-1 whole cellhuman Caco-2 cells, rat brain tissue, rat kidney tissue, rat lung tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse liver tissue, mouse NIH3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human MAD1 fragment (Leu362–Ala632), expressed in E. coli. Sequence identity with the mouse ortholog: 81%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitotic spindle assembly checkpoint protein MAD1 in the nucleus, chromosome, nucleus envelope and cytoplasm. Tissue expression noted by UniProt (human): Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level)... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
