| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA;1.14.11.-;60S ribosomal protein L27a histidine hydroxylase;Histone lysine demethylase MINA;MYC-induced nuclear antigen;Mineral dust-induced gene protein;Nucleolar protein 52;Ribosomal oxygenase MINA;ROX;MINA ;MDIG , MINA53 , NO52 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Mad2L1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MAD2L1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20M74; isotype IgG; reactivity: Human. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-Mad2L1 Rabbit Monoclonal Antibody catalog # M00785-2. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: MAD2L1 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA).
- Antibody format: Monoclonal; clone 20M74; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MAD2L1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. . Reported cellular localization context: Nucleus . Nucleus, nucleolus . Tissue expression notes (as provided): Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines. .
Research relevance and current trends
- Research context keywords from the source record include: Chromatin Modifying Enzymes,Epigenetics and Nuclear Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate MAD2L1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MAD2L1 expression by Western blot in cell or tissue lysates, Compare relative MAD2L1 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 15 kDa; calculated MW: 52800 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 15 kDa
- Cellular localization (provided): Nucleus . Nucleus, nucleolus .
- Tissue details (provided): Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
