| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mannose-6-phosphate isomerase;5.3.1.8;Phosphohexomutase;Phosphomannose isomerase;PMI;MPI;PMI1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MPI (Mannose-6-phosphate isomerase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband® catalog # A00175. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively. (reported region: A2-K99).
- Molecular weight context: reported MW: 47 kDa; calculated MW: 46656 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Mannose-6-phosphate isomerase; Mannose-6-phosphate isomerase. Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1. Functional note: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. Reported localization: Cytoplasm . Expression/tissue context: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
Research relevance and current trends
- Energy Metabolism: Researchers commonly examine how MPI (Mannose-6-phosphate isomerase) relates to this theme using model systems and orthogonal readouts.
- Energy Transfer Pathways: Researchers commonly examine how MPI (Mannose-6-phosphate isomerase) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how MPI (Mannose-6-phosphate isomerase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MPI (Mannose-6-phosphate isomerase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of MPI (Mannose-6-phosphate isomerase) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
