| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fascin-2; Retinal fascin; FSCN2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MAP4K1/HPK1 recombinant protein (Position: E30-H769). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MAP4K1/HPK1 Antibody Picoband® is an antibody for MAP4K1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MAP4K1 (fascin actin-bundling protein 2, retinal); UniProt: Q92918
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 91 kDa, calculated 49229 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MAP4K1/HPK1 Antibody Picoband® catalog # A07909-3.
Biological background
Biological context: Acts as an actin bundling protein. May play a pivotal role in photoreceptor cell-specific events, such as disk morphogenesis.
Expression and localization notes: cellular localization: Nucleus., tissue context: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina..
Common research applications
- Western blotting (WB): Compare MAP4K1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MAP4K1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify MAP4K1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Mitogen-activated protein kinase kinase kinase kinase 1 is a protein kinase that in humans is encoded by the MAP4K1 gene. It is also known as HPK1 (Hematopoietic Progenitor Kinase 1). The protein has been shown to play a role in JNK activation.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina.
- Research category: Golgi Proteins,Protein Trafficking,Signal Transduction,Vesicle Transport
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
