| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitogen-activated protein kinase 6;MAP kinase 6;MAPK 6;2.7.11.24;Extracellular signal-regulated kinase 3;ERK-3;MAP kinase isoform p97;p97-MAPK;MAPK6;ERK3, PRKM6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MAPK6 recombinant protein (Position: E520-N721). Human MAPK6 shares 86.2% and 82.8% amino acid (aa) sequence identity with mouse and rat MAPK6, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MAPK6 (Mitogen-activated protein kinase 6) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-MAPK6 Antibody Picoband® catalog # PB9666. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human MAPK6 recombinant protein (Position: E520-N721). Human MAPK6 shares 86.2% and 82.8% amino acid (aa) sequence identity with mouse and rat MAPK6, respectively. (reported region: E520-N721).
- Molecular weight context: reported MW: 100 kDa; calculated MW: 82681 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Mitogen-activated protein kinase 6; Mitogen-activated protein kinase 6. Mitogen-activated protein kinase 6 is an enzyme that in humans is encoded by the MAPK6 gene. It is mapped to 15q21. The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblastsupon treatment with serum or phorbol esters. Functional note: Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity). . Reported localization: Cytoplasm . Nucleus . Translocates to the cytoplasm following interaction with MAPKAPK5. . Expression/tissue context: Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Research relevance and current trends
- MAPK Pathway: Researchers commonly examine how MAPK6 (Mitogen-activated protein kinase 6) relates to this theme using model systems and orthogonal readouts.
- Protein Phosphorylation: Researchers commonly examine how MAPK6 (Mitogen-activated protein kinase 6) relates to this theme using model systems and orthogonal readouts.
- Ser/Thr Kinases: Researchers commonly examine how MAPK6 (Mitogen-activated protein kinase 6) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MAPK6 (Mitogen-activated protein kinase 6) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of MAPK6 (Mitogen-activated protein kinase 6) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
