Anti-MASPIN Antibody Picoband® (monoclonal, 7G4E1)

Overview

Anti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) is an antibody for SERPINB5 detection raised in Mouse (Monoclonal, clone Clone: 7G4E1, Mouse IgG2a), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SERPINB5 (cadherin 3, type 1, P-cadherin (placental)); UniProt: P36952
• Antibody format: Mouse, Monoclonal, clone Clone: 7G4E1, Mouse IgG2a
• Molecular weight: 42 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) catalog # M03409.

Biological background

Biological context: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein., tissue context: Expressed in some normal epithelial tissues and in some carcinoma cell lines..

Common research applications

• Western blotting (WB): Compare SERPINB5 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SERPINB5 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SERPINB5-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Single-pass type I membrane protein.
• Tissue details: Expressed in some normal epithelial tissues and in some carcinoma cell lines.
• Research category: ER Proteins,Immunology,Protein Trafficking,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.