| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcium-activated potassium channel subunit alpha-1;BK channel;BKCA alpha;Calcium-activated potassium channel, subfamily M subunit alpha-1;K (VCA)alpha;KCa1.1;Maxi K channel;MaxiK;Slo-alpha;Slo1;Slowpoke homolog;Slo homolog;hSlo;KCNMA1;KCNMA, SLO; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KCNMA1 recombinant protein (Position: K124-Q467). Human KCNMA1 shares 99% amino acid (aa) sequence identity with both mouse and rat KCNMA1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes calcium-activated potassium channel subunit alpha-1 (gene KCNMA1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 137.6 kDa, and the supplier reports an observed band at about 110–138 kDa.
KCNMA1 encodes the pore-forming α subunit of the large-conductance BK channel, which opens in response to both membrane depolarization and rising intracellular Ca2+. The resulting potassium efflux repolarizes the membrane and damps excitation, regulating smooth muscle tone, neurotransmitter release and frequency tuning of cochlear hair cells. BK channel properties vary with splicing, phosphorylation and accessory β subunits.
| Target | Calcium-activated potassium channel subunit alpha-1 (gene KCNMA1; UniProt Q12791, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 137.6 kDa |
| Observed MW | 110–138 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human PC-3 cells, human U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human KCNMA1 fragment (Lys124–Gln467), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places calcium-activated potassium channel subunit alpha-1 in the cell membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Liu et al. (2022) Exogenous H2S promotes ion channel reconstruction to regulate colonic motility in rats with dinitrobenzene sulfonic acid-induced colitis. Annals of Translational Medicine. 10.21037/atm-22-2126
