Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband®

Overview

This antibody is intended for detection of KCNMA1 (Calcium-activated potassium channel subunit alpha-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband® catalog # PB9227. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human KCNMA1 recombinant protein (Position: K124-Q467). Human KCNMA1 shares 99% amino acid (aa) sequence identity with both mouse and rat KCNMA1. (reported region: K124-Q467).
• Molecular weight context: reported MW: 110-138 kDa; calculated MW: 137560 MW
• Reactivity: Human,Mouse,Rat
• Applications: IHC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Calcium-activated potassium channel subunit alpha-1; Calcium-activated potassium channel subunit alpha-1. Calcium-activated potassium channel subunit alpha-1 also known as KCa1.1, or BK channel for short, is a voltage gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. This gene is located on 10q22.3. BK channels are activated (opened) by changes in membrane electrical potential and/or by increases in concentration of intracellular calcium ion (Ca2+). It is essential for the regulation of several key physiological processes including smooth muscle tone and neuronal excitability. BK channels also contribute to the behavioral effects of ethanol in the worm C. elegans under high concentrations (> 100 mM, or approximately 0.50% BAC).It remains to be determined if BK channels contribute to intoxication in humans. Functional note: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca (2+) that mediates export of K (+). It is also activated by the concentration of cytosolic Mg (2+). Its activation dampens the excitatory events that elevate the cytosolic Ca (2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca (2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Reported localization: Cell membrane ; Multi-pass membrane protein . Expression/tissue context: Widely expressed. Except in myocytes, it is almost ubiquitously expressed. .

Research relevance and current trends

• Cancer: Researchers commonly examine how KCNMA1 (Calcium-activated potassium channel subunit alpha-1) relates to this theme using model systems and orthogonal readouts.
• Cancer Metabolism: Researchers commonly examine how KCNMA1 (Calcium-activated potassium channel subunit alpha-1) relates to this theme using model systems and orthogonal readouts.
• Cardiovascular: Researchers commonly examine how KCNMA1 (Calcium-activated potassium channel subunit alpha-1) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative KCNMA1 (Calcium-activated potassium channel subunit alpha-1) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of KCNMA1 (Calcium-activated potassium channel subunit alpha-1) across tissue regions and cell types using matched controls.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the potassium channel family. Calcium- activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.