| Field | Specification |
|---|---|
| Target | |
| Alternative names | Methyl-CpG-binding domain protein 2; Demethylase; DMTase; Methyl-CpG-binding protein MBD2; MBD2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MBD2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22M01 is a rabbit monoclonal antibody that recognizes methyl-CpG-binding domain protein 2 (gene MBD2) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 43.3 kDa; the supplier reports observed bands at 43, 32 kDa.
MBD2 is a methyl-CpG-binding domain protein that binds methylated DNA and recruits the NuRD chromatin remodeling and histone deacetylase complex. Through this link, it translates DNA methylation into transcriptional repression. It is studied in epigenetic silencing of tumor suppressor genes, blood cell development and cancer.
| Target | Methyl-CpG-binding domain protein 2 (gene MBD2; UniProt Q9UBB5, human) |
|---|---|
| Clone | 22M01 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.3 kDa |
| Observed MW | 43, 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MBD2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places methyl-CpG-binding domain protein 2 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Highly expressed in brain, heart, kidney, stomach, testis and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
