| Field | Specification |
|---|---|
| Target | |
| Alternative names | Melanocyte-stimulating hormone receptor;MSH-R;Melanocortin receptor 1;MC1-R;MC1R;MSHR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MC1 Receptor. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects melanocyte-stimulating hormone receptor (gene MC1R) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 34.7 kDa, and the supplier reports an observed band at about 35 kDa.
The melanocortin 1 receptor (MC1R) is a G protein-coupled receptor on melanocytes that responds to α-MSH and ACTH. Its activation raises cAMP and shifts pigment production from red-yellow pheomelanin to brown-black eumelanin. Common MC1R variants underlie red hair and fair skin, and the receptor is studied in pigmentation, UV responses and melanoma risk.
| Target | Melanocyte-stimulating hormone receptor (gene MC1R; UniProt Q01726, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 34.7 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): PC12 cells, HEPA1–6 cells, HeLa cells, A375 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human MC1 Receptor.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places melanocyte-stimulating hormone receptor in the cell membrane. Tissue expression noted by UniProt (human): Expressed in melanocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Nan Tang et al. (2023) Restraint stress promotes monobenzone-induced depigmentation in mice via the activation of glucocorticoid receptor/macrophage migration inhibitory factor signaling pathway. Molecular Immunology. 10.1016/j.molimm.2023.07.007
