| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adrenocorticotropic hormone receptor;ACTH receptor;ACTH-R;Adrenocorticotropin receptor;Melanocortin receptor 2;MC2-R;MC2R;ACTHR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MC2 receptor, different from the related mouse sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects adrenocorticotropic hormone receptor (gene MC2R) in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 33.9 kDa, and the supplier reports an observed band at about 34 kDa.
The ACTH receptor (MC2R) is a melanocortin G protein-coupled receptor expressed mainly in the adrenal cortex. It binds adrenocorticotropic hormone and signals through cAMP to drive glucocorticoid synthesis. It needs the accessory protein MRAP to reach the cell surface, and loss-of-function mutations cause familial glucocorticoid deficiency, making it central to adrenal and stress-axis research.
| Target | Adrenocorticotropic hormone receptor (gene MC2R; UniProt Q01718, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.9 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human MCF-7 cells, human U251 cells, human HeLa cells, human K562 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Synthetic peptide from the C-terminal region of human MC2 receptor. It differs from the mouse sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places adrenocorticotropic hormone receptor in the cell membrane. Tissue expression noted by UniProt (human): Melanocytes and corticoadrenal tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
