| Field | Specification |
|---|---|
| Target | |
| Alternative names | Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT;MCL1;BCL2L3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes induced myeloid leukemia cell differentiation protein Mcl-1 (gene MCL1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 37.3 kDa, and the supplier reports an observed band at about 37 kDa.
MCL-1 is an anti-apoptotic member of the BCL-2 family that sits on the mitochondrial outer membrane. It binds and neutralizes pro-apoptotic proteins such as BAK and BIM, preventing mitochondrial outer membrane permeabilization. MCL-1 turns over rapidly, and because many cancers depend on it for survival, it is studied as a cancer target and in cell death regulation.
| Target | Induced myeloid leukemia cell differentiation protein Mcl-1 (gene MCL1; UniProt Q07820, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.3 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): rat spleen tissue, human HepG2 cells, human MCF-7 cells, human COLO320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue, human intestinal cancer tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MCL1 fragment (Met1–Arg350), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 82% and 78%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places induced myeloid leukemia cell differentiation protein Mcl-1 in the membrane, cytoplasm, mitochondrion and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Yunlong et al. (2017) Rapamycin upregulates glutamate transporter and IL-6 expression in astrocytes in a mouse model of Parkinson’s disease. Cell Death & Disease. 10.1038/cddis.2016.491
Lin Yihua et al. (2023) CYD0281, a Bcl-2 BH4 domain antagonist, inhibits tumor angiogenesis and breast cancer tumor growth. BMC Cancer. 10.1186/s12885-023-10974-4
Nianli Liu et al. (2015) Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells. FEBS Letters. 10.1016/j.febslet.2015.02.026
