| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; MCM2; BM28, CCNL1, CDCL1, KIAA0030 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MCM2 recombinant protein (Position: S393-R850). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes DNA replication licensing factor MCM2 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 101.9 kDa, and the supplier reports an observed band at about 125 kDa.
MCM2 is a subunit of the MCM2-7 complex, the ring-shaped replicative helicase that is loaded onto origins of DNA replication and unwinds DNA during S phase. MCM2 also binds histones and helps handle them at the replication fork. Because MCM proteins are present in proliferating but not resting cells, MCM2 is widely used as a proliferation marker.
| Target | DNA replication licensing factor MCM2 (gene MCM2; UniProt P49736, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 101.9 kDa |
| Observed MW | 125 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human A431 cells, human THP-1 cells, human K562 cells, mouse NIH/3T3 cells, mouse RAW264.7 cells, rat spleen tissue, rat thymus tissue, mouse SP2/0 cells, rat PC-12 cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, rat lymphatic nodules tissue, rat intestines tissue, human mammary cancer tissue, human tonsil tissue, mouse lymphatic nodules tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human MCM2 fragment (Ser393–Arg850), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA replication licensing factor MCM2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Rong Yan et al. (2024) Multi-omics reveals the role of MCM2 and hnRNP K phosphorylation in mouse renal aging through genomic instability. Experimental Cell Research. 10.1016/j.yexcr.2024.114115
