| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DNA helicase ;3.6.4.12 ;Mcm7 ;rCG_55961 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human Mcm7. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Mcm7 Antibody (Monoclonal, DCS-141) is an antibody targeting MCM7. Common applications include WB, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DCS-141; isotype: Mouse IgG1; reactivity: Human; observed MW: 42 kDa; calculated MW: 81063 MW.
Boster Bio Anti-Mcm7 Antibody (Monoclonal, DCS-141) catalog # MA1058. Tested in ICC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MCM7 — DNA helicase
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DCS-141; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 42 kDa; Calculated: 81063 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.
Scientific background (datasheet): Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.
Cellular localization (datasheet): Nucleus .
Sequence similarities (datasheet): Belongs to the MCM family.
Research relevance and current trends
- Commonly studied in contexts related to DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
