| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA helicase MCM8; DNA 3'-5' helicase MCM8; Minichromosome maintenance 8; MCM8; C20orf154 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MCM8 recombinant protein (Position: N598-M840). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA helicase MCM8 in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 93.7 kDa, and the supplier reports an observed band at about 94 kDa.
MCM8 is a helicase related to the replicative MCM proteins but not part of the MCM2-7 complex. Together with MCM9, it forms a helicase that supports homologous recombination repair of DNA double-strand breaks and interstrand cross-links, partly by helping the MRN complex resect DNA ends. Mutations in MCM8 are linked to primary ovarian insufficiency and infertility.
| Target | DNA helicase MCM8 (gene MCM8; UniProt Q9UJA3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 93.7 kDa |
| Observed MW | 94 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human placenta tissue, rat testis tissue, rat H9C2(2–1) cells, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human MCM8 fragment (Asn598–Met840), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA helicase MCM8 in the nucleus, chromosome, cytoplasm and mitochondrion matrix. Tissue expression noted by UniProt (human): Highest levels in placenta, lung and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
