| Field | Specification |
|---|---|
| Target | |
| Alternative names | Malate dehydrogenase, cytoplasmic; Aromatic alpha-keto acid reductase; KAR; Cytosolic malate dehydrogenase; MDH1; MDHA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MDH1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23M24 is a rabbit monoclonal antibody that recognizes malate dehydrogenase, cytoplasmic (gene MDH1) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 36.4 kDa, and the supplier reports an observed band at about 36 kDa.
MDH1 is the cytoplasmic malate dehydrogenase, which interconverts malate and oxaloacetate using NAD+/NADH. It is a key part of the malate-aspartate shuttle that moves reducing equivalents from the cytosol into mitochondria, and it also supports gluconeogenesis. MDH1 is studied in cellular energy metabolism and cancer metabolism.
| Target | Malate dehydrogenase, cytoplasmic (gene MDH1; UniProt P40925, human) |
|---|---|
| Clone | 23M24 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.4 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MDH1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places malate dehydrogenase, cytoplasmic in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
