Anti-ME1 Antibody Picoband®

Overview

Anti-ME1 Antibody Picoband® is an antibody for ME1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: ME1 (solute carrier family 31 member 1); UniProt: P48163
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 64 kDa, calculated 64 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-ME1 Antibody Picoband® catalog # A03449-3.

Biological background

Biological context: High-affinity, saturable copper transporter involved in dietary copper uptake.

Expression and localization notes: cellular localization: Cell membrane; Localizes to the apical membrane in intestinal epithelial cells., tissue context: Found in the synovial fluid of patients with rheumatoid arthritis. ..

Common research applications

• Western blotting (WB): Compare ME1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of ME1 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify ME1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane; Localizes to the apical membrane in intestinal epithelial cells.
• Tissue details: Found in the synovial fluid of patients with rheumatoid arthritis. .
• Research category: Neuroscience,Neurotransmission,Receptors / Channels

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.