| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Melanocyte protein PMEL; Melanocyte protein Pmel 17; Premelanosome protein; Silver locus protein; M-alpha; M-beta; Pmel; D10H12S53E; Pmel17; Si; Silv |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived mouse Melanoma gp100/Pmel recombinant protein (Position: S27-V626). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Melanoma gp100/Pmel Picoband® Antibody is an antibody for Pmel detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Pmel (premelanosome protein); UniProt: Q60696; NCBI Gene: 20431
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 95 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Melanoma gp100/Pmel Picoband® Antibody catalog # A01262-2.
Biological background
Biological context: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures.
Expression and localization notes: cellular localization: Multivesicular body. Golgi apparatus. Endoplasmic reticulum membrane. Single-pass type I membrane protein. Melanosome., tissue context: Ubiquitous..
Common research applications
- Western blotting (WB): Compare Pmel levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Pmel in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Pmel-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Melanocyte protein PMEL also known as premelanosome protein (PMEL) or silver locus protein homolog (SILV) is a protein that in humans is encoded by the PMEL gene. It is mapped to 10 D3; 10 77.13 cM. This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Multivesicular body. Golgi apparatus. Endoplasmic reticulum membrane. Single-pass type I membrane protein. Melanosome.
- Tissue details: Ubiquitous.
- Research category: Amino Acid Metabolism,Amino Acids,Cancer,Cell Type Markers,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction,Tumor Associated,Tumor Immunology,Tumor-Associated Antigens
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
