| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase Mer;2.7.10.1;Proto-oncogene c-Mer;Receptor tyrosine kinase MerTK;MERTK;MER; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MERTK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone COI-13 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase Mer (gene MERTK) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 110.2 kDa, and the supplier reports an observed band at about 188 kDa.
MER (MERTK) is a receptor tyrosine kinase of the TAM family (TYRO3, AXL, MER). Activated by the bridging ligands GAS6 and protein S, it drives phagocytic clearance of apoptotic cells by macrophages and of photoreceptor outer segments by the retinal pigment epithelium, and it dampens inflammation. MERTK mutations cause retinitis pigmentosa, and it is studied in immunology and cancer.
| Target | Tyrosine-protein kinase Mer (gene MERTK; UniProt Q12866, human) |
|---|---|
| Clone | COI-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 110.2 kDa |
| Observed MW | 188 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lymphoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human MERTK.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein kinase Mer in the cell membrane. Tissue expression noted by UniProt (human): Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
