| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mesothelin;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;Msln;Mes, Mpf; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets mesothelin (gene Msln) in mouse samples and is validated for IHC. The predicted molecular weight is 69.4 kDa.
Mesothelin (MSLN) is a GPI-anchored glycoprotein normally found on mesothelial cells that line the pleura, peritoneum and pericardium. It is made as a precursor that is cut into membrane-bound mesothelin and a secreted fragment called megakaryocyte-potentiating factor, and it is widely studied because it is highly expressed in mesothelioma and in pancreatic and ovarian cancers.
| Target | Mesothelin (gene Msln; UniProt Q61468, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 69.4 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
|---|
Samples with confirmed staining (IHC): mouse lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Mesothelin fragment (Lys308–Leu576), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places mesothelin in the cell membrane and Golgi apparatus. Tissue expression noted by UniProt (mouse): Highly expressed in lung and heart.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.