| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mesothelin;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;Msln;Mes, Mpf; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Msln (Mesothelin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Mesothelin/MSLN Antibody Picoband® catalog # PB9671. Tested in IHC applications. This antibody reacts with Mouse.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively. (reported region: K308-L576).
- Molecular weight context: reported MW: 54 kDa; calculated MW: 69423 MW
- Reactivity: Mouse
- Applications: IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Mesothelin; Mesothelin. Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Functional note: Membrane-anchored forms may play a role in cellular adhesion. . Reported localization: Cell membrane ; Lipid-anchor, GPI-anchor . Golgi apparatus . Expression/tissue context: Highly expressed in lung and heart. Expressed at low levels in spleen, liver, kidney and testis. Present in lung (at protein level). .
Research relevance and current trends
- Cancer: Researchers commonly examine how Msln (Mesothelin) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how Msln (Mesothelin) relates to this theme using model systems and orthogonal readouts.
- Tags & Cell Markers: Researchers commonly examine how Msln (Mesothelin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of Msln (Mesothelin) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
