| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte growth factor receptor;HGF receptor;2.7.10.1;HGF/SF receptor;Proto-oncogene c-Met;Scatter factor receptor;SF receptor;Tyrosine-protein kinase Met;MET; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Met (c-Met) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BFA-13 is a rabbit monoclonal antibody that recognizes hepatocyte growth factor receptor (gene MET) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 155.5 kDa, and the supplier reports an observed band at about 155 kDa.
MET is the receptor tyrosine kinase for hepatocyte growth factor (HGF). HGF binding activates MET and downstream pathways such as RAS-MAPK and PI3K-AKT, promoting epithelial cell scattering, migration, proliferation and survival during development and tissue repair. MET amplification, mutation or overexpression occurs in many cancers, making it a major target in cancer research.
| Target | Hepatocyte growth factor receptor (gene MET; UniProt P08581, human) |
|---|---|
| Clone | BFA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 155.5 kDa |
| Observed MW | 155 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): human stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human met (c-Met).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hepatocyte growth factor receptor in the membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhihao Li et al. (2026) Single-cell transcriptomics reveals mechanisms of Galt gene editing–induced liver injury involving HGF–VEGF–mediated intercellular signaling in mice. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2025.1729321
Ying Yang et al. (2009) Potential treatment of liver-related disorders with in vitro expanded human liver precursors. Differentiation. 10.1111/j.1432-0436.2007.00184.x
