| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Methylmalonyl-CoA mutase, mitochondrial;MCM;5.4.99.2 ;Methylmalonyl-CoA isomerase;MUT; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MUT (Methylmalonyl-CoA mutase, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® catalog # A01065. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.
- Molecular weight context: reported MW: 83 kDa; calculated MW: 83 kDa
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Methylmalonyl-CoA mutase, mitochondrial; Methylmalonyl-CoA mutase, mitochondrial. Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle. Functional note: Involved in the degradation of several amino acids, odd- chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species. Reported localization: Mitochondrion matrix. Expression/tissue context: T-cell specific.
Research relevance and current trends
- Apoptosis: Researchers commonly examine how MUT (Methylmalonyl-CoA mutase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how MUT (Methylmalonyl-CoA mutase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Cancer Susceptibility: Researchers commonly examine how MUT (Methylmalonyl-CoA mutase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MUT (Methylmalonyl-CoA mutase, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of MUT (Methylmalonyl-CoA mutase, mitochondrial) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
