| Field | Specification |
|---|---|
| Target | |
| Alternative names | MAX gene-associated protein;MAX dimerization protein 5;MGA;KIAA0518, MAD5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MGA, different from the related mouse sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets MAX gene-associated protein (gene MGA) in human samples and is validated for Western blot. The predicted molecular weight is 336.2 kDa, and the supplier reports an observed band at about 334 kDa.
MGA (MAX gene-associated protein) is a large transcription factor that contains both a T-box and a bHLH-zip domain. It dimerizes with MAX to bind E-box sequences and generally represses MYC target genes, and it is part of a non-canonical Polycomb (PRC1.6) complex. MGA is frequently inactivated in some cancers and is studied as a MYC antagonist.
| Target | MAX gene-associated protein (gene MGA; UniProt Q8IWI9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 336.2 kDa |
| Observed MW | 334 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells, MCF-7 cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human MGA. It differs from the mouse sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places MAX gene-associated protein in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in germ cells and granulosa cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
