| Field | Specification |
|---|---|
| Target | |
| Alternative names | HLA class II histocompatibility antigen, DP beta 1 chain;HLA class II histocompatibility antigen, DP (W4) beta chain;MHC class II antigen DPB1;HLA-DPB1;HLA-DP1B; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MHC Class II |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAEB-8 is a rabbit monoclonal antibody that recognizes HLA class II histocompatibility antigen, DP beta 1 chain (gene HLA-DPB1) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 29.2 kDa, and the supplier reports an observed band at about 29 kDa.
HLA-DPB1 encodes the β chain of HLA-DP, one of the classical MHC class II molecules. Paired with its α chain on antigen-presenting cells, it displays peptides from proteins taken up by endocytosis to CD4+ helper T cells. The gene is highly polymorphic and is typed in transplantation and immunogenetic studies.
| Target | HLA class II histocompatibility antigen, DP beta 1 chain (gene HLA-DPB1; UniProt P04440, human) |
|---|---|
| Clone | AAEB-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.2 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MHC Class II.
Reactivity Notes
The supplier lists reactivity with human. UniProt places HLA class II histocompatibility antigen, DP beta 1 chain in the cell membrane, endoplasmic reticulum membrane, endosome membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
